TIWB Manual: Tips

General

How to get numeric tables of data.

TI Workbench supplies creating plots for time course of image intensity or electrophysiological data. When numerical tables are needed for processing on spread sheet software, etc., they can produced on Igor. Exporting data to Igor makes plots on Igor. At this point, Igor already has data table inside. Just selecting "tables..." in "Window" menu of Igor lets you make data table on Igor, which can be saved as a text file or copied to clipboard.

Time course of segment of images can be saved as a text file (creator is Excel), through "Export..." in the "File" menu.

How to remove unnecessary data segments from a file.

This can be done by making a copy of the file containing only necessary segments. refer here.

Image Off-line

Parameter files (".tiprm") are not created which should be when foreign format image files are opened.

".tiprm" files are created in the same folder as the data files. If the folder is write-protected, or the "save back parameters" option in the "imageOption(Global)" is not checked, parameter files are not created.

How can fluorescence changes of moving cells can be tracked in time-course plot.

By pressing the option key or the option + control keys, shapes and positions of ROIs can be changed in a segment of frames. Refer to here.

Shaded boxes and dotted lines in exported Igor graph can not be changed or deleted.

Shaded boxes in exorted time course plot in Igor graph indicates baseline range or analyze area. A dotted line in the graph indicates the time point of the accompanying image frame. These shaded boxes and dotted lines can not be changed or removed in Igor Graph window, because they are drawn in "User Back" layer. The default layer is "User Front". To change the layer in Igor Graph, do the following.

Activate Igor Graph window.
Choose "Graph" -> "Show Tools"
Click "Pallete" icon to show drawing tools in the left side of the Graph window.
Press "Draw Environment" icon (second one from the bottom of the icons) with pressing the "option" key. And choose "User Back" in the pop-up menu.

Shaded boxes are drawn in the "User Back" layer, otherwise the boxes hide plots.

Electrophysiology On-line

Seal formation in patch clamp recording.

To monitor seal formation in patch clamp recording, use "Peak Detection" function. With peak detection check box in the Oscillo graph on and applying square pulses as voltage commands by "auto trigger" check box in the Oscillo Panel on, resulting current step size is displayed in the Peak Detection Dialog.

Electrophysiology Off-line

How to make an I-V plot.